Benefits of Custom Gene Synthesis
The advancement of technology and science has been critical in providing humanity with a higher quality of life. Every advancement in science has benefited humankind in numerous ways, from the invention of new pharmaceuticals to the creation of advanced healthcare and diagnosis equipment. Regarding genetics research, gene synthesis is one of the essential techniques utilized by scientists worldwide to focus on various applications, including therapeutic antibody engineering, vaccine design, plant biology, genetics, biochemistry, and many more.
If we define gene synthesis scientifically, it is the synthetic biology approach for developing artificial genes in a laboratory context. Hard Gobind Khorana and friends completed this technique in 1972; since then, this technology has grown in popularity. Gene synthesis is currently well-known for its effectiveness, efficiency, and popularity. The ability to create large sequences of genes or DNA, over 150 Kb, with great accuracy is one of the reasons for gene synthesis’s popularity. This ability to generate vast sequences of genes opens up many possibilities for research into issues like synthetic DNA library production.
Furthermore, gene synthesis has been used in various biological studies and genetics due to its excellent precision and extended length. Below are the benefits of Custom Gene Synthesis.
Why Is Gene Synthesis Important?
Gene synthesis can create double-stranded DNA in vitro, with an assembly capacity of 50bp to 12Kb products, which is not conceivable with classical molecular cloning and PCR cloning, thanks to advances in genetic research. It’s a complicated process that professionals should do for a quality outcome. You can get professional assistance by clicking Custom Gene Synthesis. Microbiological procedures are often utilized in classical molecular cloning to construct recombinant DNA molecules and perform replication within host organisms. However, it is impossible to guarantee that every gene will have high-efficiency expression during this process. Significantly, gene synthesis is the most excellent method for cloning and genetic alterations since it is an entirely new system that delivers high-efficiency expression of the target gene.
Future Landscape of the Gene Synthesis Market
The global gene synthesis market is predicted to rise steadily soon, thanks to the growing use of gene synthesis in healthcare worldwide. During the analysis period of 2021-2028, the market is expected to produce revenue of $42,14,890.1 thousand and increase at a CAGR of 18.5 percent. Furthermore, the growing population of people with hereditary and chronic diseases is projected to open up a lot of new business prospects soon.
Applications of gene synthesis
- Different mutant genes, SNPs, or other mutants are synthesized.
- Large-scale synthesis of microchip cDNA.
- Antibodies cloned from humans or mice, as well as recombinant antibodies.
- Different mutant genes, SNPs, or other mutants are synthesized.
Various Gene Synthesis Methods
You cannot substitute the many gene synthesis methods for one another; each process is designed to meet distinct needs. Outlined are some of the most prevalent gene synthesis methods:
PCR-based Enzyme Synthesis: Gene fragments are produced in this procedure using various cell systems. Multiple genes are inserted into the main strands during the process, allowing the target gene to be as long as it wants. This method aids in synthesizing large gene segments, and using a coalition of cell systems ensures excellent precision.
Chip-based DNA Synthesis: This gene manufacturing approach is carried out with the use of microarray chips and a variety of electrochemical techniques. In this process, different oligonucleotides are generated in several sections of the chip called assembly sub-pools. These sub-pools are made of many gene fragments that are magnified, aggregated, and combined to form the primary product.
Solid-based Synthesis: It is a conventional gene synthesis method that uses a tiny amount of solution treated in a chemical column. Coupling, de-blocking, capping, and oxidation are the four phases of this process. For products larger than 200bp, the sequence coalition and synthesis productivity are constrained. As a result, the length of the DNA sequence obstructs this process. The cost of this method is higher than that of chip-based DNA synthesis. The greatest benefit of this system, however, is its excellent precision.
Advantages of Gene Synthesis
Gene synthesis provides several benefits, some of which are listed below:
- It has a quick synthesis cycle time and excellent precision.
- Researchers can undertake numerous research activities with the help of gene synthesis since it provides a broader scope for extracting genes that are difficult to obtain.
- Gene synthesis allows for more flexibility in changing gene sequences to facilitate diverse cloning operations.
- Most crucially, gene synthesis can modify all of the target codons at the same time. As a result, there is no need to modify the expressions of the target gene frequently.
- Gene Synthesis reduces the amount of time and effort required by humans.
Bottomline
The need for genetic synthesis is expected to rise due to the increased number of individuals suffering from chronic diseases such as cancer. Furthermore, with the outbreak of the Covid-19 pandemic, the genetic synthesis industry is expected to strengthen in the coming days due to increased investments by governments, private and public enterprises in the field of synthetic biology, as well as increased demand for making vaccines.